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文献和实验. subtilis ), whereas others such as E. coli are not naturally competent. Non-competent cells can be made competent and then transformed via one of two main approaches; chemical transformation and electroporation . There are advantages and disadvantages
Inducible shRNA Lentiviral Vectors
Stbl3™ Competent E. coli Note: You may store the LR reaction at -20° C for up to 1 week before transformation, if desired. 2. Transforming One Shot® Stbl3™ Competent E. coli 1) Thaw, on ice, one vial of One Shot® Stbl3™ chemically
efficiency is sufficient, we use heat shock transformation and chemically competent cells. The choice of the E. coli host strain depends on the goal of the transformation. ·The transformation of a ligation mix should be done in a recA- cloning strain
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