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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
, during time series analyses or when screening transfor-mants for expression of a transformed gene. Under such circumstances, existing tech-niques are overly time consuming and yield more RNA than is necessary. The availability of a rapid RNA mini-prep
. Resuspend bacterial pellet in 350 µl of STET buffer. Add 25 µl of freshly prepared solution of lysozyme (10 mg/ml in 10 mM Tris-Cl, pH 8). Mix by vortexing for 3 sec. Place the tube in a boiling water bath for exactly 40
Pellet 1.5 ml of an overnight culture at 12,000 rpm in Eppendorf centrifuge at RT for 3 min. Resuspend bacterial pellet in 350 µl of STET buffer. Add 25 µl of freshly prepared solution of lysozyme (10 mg/ml in 10 mM Tris-Cl, pH 8). Mix
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