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文献和实验Immune Response Biomarker Profiling
实验试剂 ProtoArray® Human Protein Microarray v5.0 Blocking Buffer and Washing Buffer 10 × Synthetic Block [ 详细信息： Invitrogen ] Alexa Fluor® 647 Goat Anti-Human IgG [ 详细信息： Invitrogen ]
Western Blotting with Monoclonal Ant
the enzyme conjugate anti-mouse IgG:HRPO (Catalog# M15345) 1:2000 in wash buffer containing 5% non-fat dry milk (or 1% BSA for phosphotyrosine antibodies). 3.Decant the wash buffer, add the diluted enzyme conjugate and incubate with agitation for 30 minutes
Western Blotting with Monoclonal Antibodies
for 30 minutes with agitation, changing the wash buffer every 3? minutes. Dilute the enzyme conjugate anti-mouse IgG:HRPO (Catalog# M15345) 1:2000 in wash buffer containing 5% non-fat dry milk (or 1% BSA for phosphotyrosine antibodies).
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