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上海玉博生物科技有限公司
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文献和实验. no.: 6 - 6201 - 004),內含 : 4x Fab-TACS® Gravity column (capacity pro column: 15 ml whole blood) 1x CD3 Fab-Strep, human 1x Buffer CI (10x) 1x Biotin stock solution 1x Fab-TACS® Gravity Adapter Fab-TACS® Flow Restrictor, Pack of 5 1.1 用 ddH2O 将 10
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
Cell Surface Immunofluorescence Staining Protocol
-CD4-Biotin, and anti-CD8-APC) and incubate on ice for 15-20 minutes in the dark. 9) Wash 2X with at least 2 ml of Cell Staining Buffer by centrifugation at 350 x g for 5 minutes. 1) If using a purified primary antibody, resuspend
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