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- 详细信息
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上海玉博生物科技有限公司
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文献和实验(Invitrogen) at a 1:3 ratio (e.g. 1µl plasmid to 3µl lipofectamine). The plasmid and lipofectamine are mixed together, vortexed, pulse spun, and then 50µl of DMEM (without Fetal bovine serum, FBS) added. The entire mixture is again vortexed followed by a pulse
(or [alpha-32P]-dCTP)** (1.85MBq = 50µCi) 6µl Klenow Fragment, exo- (5u) 1µl 4.Shake the tube and spin down in a microcentrifuge for 3-5sec. Incubate for 10min at 37℃. 5.Add
Construction and Characterization of Adenovirus Vectors
the number of plaques in a well by the dilution factor to determine the vector titer (pfu/mL). To determine vector titer in particles/milliliter: x. Dilute 20-50 µL of purified vector to a final
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