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文献和实验In this chapter we describe the methods currently used for subgrouping Legionella pneumophila and other non-pneumophila species. In the first part we describe monoclonal antibody (mAb) subgrouping, either by indirect immunofluorescence
Characterization of Legionella Lipopolysaccharide
The lipopolysaccharide(LPS) of Legionella spp. is an immuno-dominant antigen and the basis for Legionella pneumophila serogroup classification. The LPS shows a peculiar structure composed of a very hydrophobic lipid A acylated by long chain
Identification of Legionella in Clinical Samples
, although this test is limited to L. pneumophila serogroup 1 (Lp1) (Helbig et al., J Clin Microbiol 41:838–840, 2003). The use of molecular techniques can improve Legionaire’s disease (LD) diagnosis by detecting other serogroups and species (Diederen et al., J Clin
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