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文献和实验Fix cells in 2% formaldehyde in PBS/pH 7.4 for 15 min. at 20oC. 2% formaldehyde is made up fresh prior to use by dissolving the appropriate amount of EM grade paraformaldehyde (Prill form, from Electron Microscopy Sciences) in PBS
免疫荧光技术--Immunofluroescence Technique
Fix cells in 2% formaldehyde in PBS/pH 7.4 for 15 min. at 20oC. 2% formaldehyde is made up fresh prior to use by dissolving the appropriate amount of EM grade paraformaldehyde (Prill form, from Electron
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of enough indigo to make it through the various solvents required for embedding for electron microscopy (EM) (Snyder et al. 1992). Double staining of cells for ß-gal with X-gal and a cellular antigen using antibodies is difficult, though possible (Snyder et
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