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文献和实验Yeast mini whole cell extract using glass beads for western analysis
from this point on. 3. Resuspend cells in 600 microliters extraction buffer and transfer to 13 x 100 mm glass test tubes on ice. Add 400 microliters glass beads. 4. In the cold room, vortex for 1 min on ice and keep on ice for at least 1 min
once with 20 ml Pbs transfer to 2 ml Eppendorf tube, wash again with Pbs and freeze cells or proceed on resuspend in 200 - 400 µl CHIP lysis buffer , add an equal volume of glass beads shake for 30 min at 4 °C on vortexer (maximum
Construction and Characterization of Adenovirus Vectors
C. Cells: A549 Cells: 293, low-passage Grow 293 and A549 cells in 150-mm dishes in a 37°C, 5% CO2 incubator and split 1:2 or 1:3 when they reach ~90% confluence. Cells: 293N3S Maintain 293N3S cells in 150-mm dishes
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