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文献和实验Immunofluorescent staining of Sea Urchin embryos
at room temperature. Embryos will settle. 3. Remove most of liquid. Add 1 ml SW to wash. Allow to settle, remove liquid. 4. Add 100 ul Fluorescein- or Texas red-congugated anti-mouse IgG (diltuted according to manufacturer's recommendations
-20 in 50 mM Na-phosphate buffer, pH 7.3. Goat-anti-mouse antibodies labeled with AlexaFluor-488 were from Invitrogen, Inc. (dye/protein ratio 7). Microscope glass slides, 3 × 1 in., and 1 mm thick, were from VWR. Water was purified by Milli-Q
STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN
antibodies against Gc-globulin (DAKO A021), diluted 1:500 with PBS/BSA. 5. Secondary goat anti-rabbit antibodies, typically diluted 1:100. Procedure 1. Fix and extract cells following the formaldehyde-acetone protocol . 2. Wash the coverslip
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