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文献和实验Transient transfection into 293T cel
), NEAA, Na-pyruvate, and Gln (Pen/Streptmycin: optional)• Transfection reagents:i) 2M CaCl2 : 2M in H2 O, filter sterile, store at 4°Cii) x2HBS: 8.0g NaCl, 0.37g KCl, 201mg (yes! mg) Na2 HPO4 •7H2 O, 1.0g glucose, 5.0g HEPES/500ml (adjust pH to 7.05
cells/100 mm dish/150 cm2 flask; 0.5 ml per 5 x106 cells/60 mm dish/75 cm2 flask). 3.Scrape adherent cells off the dish or flask with either a rubber policeman or a plastic cell scraper that has been cooled in ice-cold distilled water.Transfer the cell
buffer to cells (1 ml per 107 cells/100 mm dish/150 cm2 flask; 0.5 ml per 5 x 106 cells/60 mm dish/75 cm2 flask). 3. Scrape adherent cells off the dish or flask with either a rubber policeman or a plastic cell scraper that has been cooled in ice-cold
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