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文献和实验CNV Analysis Using TaqMan Copy Number Assays
‐designed or custom assays; Applied Biosystems): store at –15° to –25°C, protected from light, minimize freeze‐thaw cycles by diluting 60× stocks to 20× working stock and dispensing into smaller volumes (recommended
, 5% CO2 incubator at 37ºC until they are 90%-95% confluent. Change the medium every 2 d. 20. Passage the cells of each clone into one well of a gelatin-coated, 12-well sterile tissue culture plate. Use a final per-well volume of 1 mL
The Mouse as Model System to Study Host‐Pathogen Interactions in Influenza A Infections
. 2009. Transmission and pathogenesis of swine‐origin 2009 A(H1N1) influenza viruses in ferrets and mice. Science 325:484‐487. Matsuoka, Y., Lamirande, E.W., and Subbarao, K
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