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上海玉博生物科技有限公司
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文献和实验Immunohistochemisty-Enzymatic Protocol
-fix tissue sections on the slide before starting immunohistochemical staining. To recover antigens in over-fixed tissues, either protease-induced epitope retrieval (PIER) or heat-induced epitope retrieval (HIER) techniques are recommended. HIER
Preparation and Staining of Paraffin Sections
in 3% H2 O2 solution in methanol for 10 minutes. Rinse in PBS 2X for 5 minutes each time. If the antibody staining requires antigen retrieval to unmask the antigenic epitope refer below to section C. If antigen retrieval is not required
Immunohistochemistry / Immunocytochemistry Procedures
is maximized. The most common techniques are enzymatic digestion or heat induced epitope retrieval (HIER) through microwave irradiation, autoclaving or pressure cooking. Enzymatic Digestion This technique involves
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