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文献和实验Studies of the Ubiquitin Proteasome System
) Kit for first‐stand cDNA synthesis (e.g., RT‐PCR kit; Fermentas) Kit for PCR amplification of DNA (e.g., RT‐PCR kit, Fermentas, or Ready‐To‐Go PCR beads, GE Healthcare) Primers for PCR
of characterized zinc-finger domains allowed the prediction of potential DNA binding sites for each TFZF after the functional screen or selection was done. We created the 3ZF library by combinatorial assembly of three different zinc-finger repertoires (ZF
Evaluation of Proliferation of Neural Stem Cells In Vitro and In Vivo
techniques to evaluate cell proliferation based on EdU incorporation: (a) immunocytochemistry and (b) flow cytometry. EdU, a new thymidine analog, which is detected by a reproducible and sensitive method based on click chemistry, does not require DNA
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