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文献和实验Dual- and Triple-Color Fluorospot
. Spontaneous production is determined by incubating the same number of cells in the absence of antigen. A polyclonal activator, such as anti-CD3 mAb (100 ng/ml) or phytohemagglutinin (PHA; 1–10 μg/ml), should normally be used as a control for cell viability
Clonality Detection of Expanded T-Cell Populations in Patients With Multiple Myeloma
are monoclonal or biclonal, whereas the CD57- cells are usually polyclonal.
Intracellular Immunofluorescent Staining for Flow Cytometry
: Intracellular Staining Quick Guide Non-Cytokine Proteins: Intracellular Staining Quick Guide Antigen Antibody Mouse/Rat Bcl-2 10C4 Mouse CTLA-4 (CD152) UC10-4B
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