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文献和实验电镜样品制备方法 Specimen preparation for electron microscopy
into approx. 1mm cubes for fixing. This may be done in a drop of fix on a sheet of dental wax, using a razor blade. Place in glass processing vials and close with plastic caps. (Tissue may be stored at this stage.) Wash in 0.1M buffer - 1 hour x
on a sheet of dental wax, using a razor blade. Place in glass processing vials and close with plastic caps. (Tissue may be stored at this stage.) Wash in 0.1M buffer - 1 hour x 2. (Or overnight at 4o C.) Post fix in osmium tetroxide in 0.2
Expression Cloning of Neural Genes Using Xenopus laevis Oocytes
2.4–cm diameter DE81 filters (Whatman) Scintillation fluid and vials
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