GE 代理商|RPN203B HYBOND-N+, 20CM

另一种BSA——N,O-双三甲硅基乙酰胺

相关专题 它们都是BSA N,O-Bis(trimethylsilyl)acetamide [BSA ] 别名：N,O-双(三甲基硅基)乙酰胺 CAS No： 10416-59-8 分子 式：C8H21NOSi2 分子量：203.43 通用名称：BSA 性状： 无色至浅黄色液体，b.p ,74-76oC,fp42oC,d420 0.829,nd20 1.417

Southern Blot using Neutral Transfer

-Elaine Pinheiro and Aurora Burds Connor MIT, Jan2006 This protocol works very well with Hybond-XL membrane from GE Healthcare (formerly Amersham). 1) Digest your genomic DNA, using about 10 μg per reaction in 100 μl total digestion

小RNA的Northern BLOT

ml DEPC H2O~1.8% dye. Mix 95 uL Formamide with 5 uL 1.8% Bromophenolblue/Xylene cyanolFF Note: can use less dye. Hybond N+: Amersham RPN303B ULTRAhyb-Oligo Hybridization Buffer: Ambion cat # 8663, keep in refrigerator T