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上海玉博生物科技有限公司
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文献和实验DNeasy 96植物技术:使用搅拌磨MM 300从新鲜植物叶片中分离DNA
a rack of collection microtubes without samples or buffers, but containing tungsten carbide beads, and fixing this second sandwich into the empty clamp (本方法用来对2×96=192个样本进行提取DNA ,如果希望处理96个或更少样本,要对搅拌磨MM 300进行平衡处理,方法是用一架没有样本和缓冲液但装有碳化钨珠子的收集微型管插入第二个孔板,并用空夹子加入
E-Z 96® M13 Isolation Vacuum Manifold Protocol
and incubate for 10 minutes to completely dry the plate. 14. Re-assemble the vacuum manifold by now replacing the waste collection tray with a 1.2 mL rack of microtubes (supplied). Place the E-Z® 96 DNA Plate on the top part of the manifold. 15. Add
In Situ HYBRIDIZATION TO TISSUE SECTIONS
In Situ HYBRIDIZATION TO TISSUE SECTIONS (Kornberg et al., Cell 40:45, 1985)) PARAFFIN SECTIONS Preparation of embryos (¾10 hr) for paraffin sections Wash embryos off collection plates onto a nylon screen with 0.4% NaCl
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