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文献和实验Increasing Sensitivity in Northern Analysis with RNA Probes
and straightforward procedure. Virtually all modern cloning vectors have one or more bacteriophage promoters (T7, T3, or SP6) outside the multiple cloning site. To prepare these plasmid templates for in vitro transcription of the antisense strand (for hybridization
The In Situ PCR:Amplification and Detection in a Cellular Context
must be retained within the permeabilized cell for the detection by radiolabelled probes; this necessitates a relatively large size for the designed product DNA, which is contrary to the relatively low efficiency for the in situ amplification reaction
by radiolabelled probes; this necessitates a relatively large size for the designed product DNA, which is contrary to the relatively low efficiency for the in situ amplification reaction. All of these considerations amplify the overall problem of detecting PCR
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