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文献和实验Retroviral Gene Expression Control in Primary Organoid Cultures
) Infection medium (see recipe ) with and without polybrene TrypLE (Invitrogen) Organoid culture
Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
β-mercaptoethanol, 1000X Gibco® Mouse Embryonic Fibroblasts (Irradiated) Basic Fibroblast Growth Factor (bFGF) GlutaMAX™-I (100X) Collagenase Type IV for enzymatic passaging or StemPro® EZPassage™ Tool for mechanical
Primary Mouse Small Intestinal Epithelial Cell Cultures
for optimal growth factor combinations based on genetic evidence of self-renewal regulation, differentiation, and carcinogenesis of intestinal stem cells. Here, we describe methods that we have established for the isolation and culture of primary small
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