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文献和实验COMPARISION OF DIFFERENT PROTEIN DETERMINATION METHODS
(sometimes you can normalize using same detergent concentration in blank and standarts ) PIERCE (#23200) SIGMA (#610-A) Coomassie Protein Assay (pdf) BRADFORD 1-1500 µg/ml
Isolate It All:siRNA • miRNA • Total RNA • Native Protein
or by solution hybridization assay with the mir Vana™ miRNA Detection Kit (Ambion). RNA probes were prepared by in vitro transcription (mRNA probes--MAXIscript® Kit, Ambion) or by 5' end labeling (rRNA, miRNA and siRNA probes - mir Vana™ Probe & Marker Kit
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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