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文献和实验Models of Inflammation: Carrageenan‐ or Complete Freund Adjuvant (CFA)
cutaneous hypersensitivity. This unit details methods to elicit and measure carrageenan? and complete Freund's adjuvant (CFA)?induced cutaneous inflammation. Due to possible differences between the dorsal root sensory system and the trigeminal sensory
to make a master mix of everything (including the enzyme - it''s thermal stable) except the template. Aliquot the mixture into tubes, and add the template just prior to putting them in a thermal cycler. Other precautions include the use of cotton plugged
. It then should be transferred to decalcification (11% formic acid with stirring) solution for overnight prior to paraffin processing. One femur should be saved for cytology as well. With a 26G needle flush 1 mL of media (Iscove's +15% heat inactivated serum) through the marrow
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