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文献和实验The Peroxidase-Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods
and indirect techniques, while still used today, were found to be less sensitive than the later-used peroxidase-antiperoxidase (PAP) techniques. The newer immune complex polymer type of detection system (Envision™ Dako Corporation, Santa Barbara, CA) increases
v468 Chapter 7 通过免疫组化来测定β-连环蛋白核定位
buffer. 9. Polymer horseradish peroxidase (HRP)-labelled anti-mouseEnvision (Dako, Glostrup, Denmark) (see Note 2 ). 10. Diaminobenzidine (DAB) peroxidase substrate. To makeup 10× DAB, dissolve 600 mg DAB (Sigma, St. Louis,MO) in 100 mL distilled
2 (1); ERBB3 (1); FLT3 (1); FLT4 (1); FLT5 (1); HRAS (2); JAK3 (3); KIT (3); KRAS (5); MET (5); MLH1 (1); MYC(6); PDGFRA (11); PIK3CA (5); PTEN (14); RB1 (11);RET (13); SRC (1); STK11 (12); P53 (7); VH1 (7) MelaCarta™
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