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E.Z.N.Z.TM MicroElute RNA Clean-up Protocol

upon addition of ethanol in Step 3. Vortex and add the entire mixture to the column. With the spin column inside a 2ml collection tube (supplied with kit), centrifuge at 10,000 x g for 30 seconds at room temperature. Discard flowthrough and re-use the collection

E-Z 96® M13 Isolation Vacuum Manifold Protocol

9. Next, apply the vacuum until all of the liquid passes through the membrane. 10. Add 1 mL of SPW Wash Buffer into each well of the E-Z 96® DNA Plate and switch on the vacuum until all of the liquid has passed through the membran. 11. Remove the E-Z

E.Z.N.Z.TM MicroElute RNA Desalting and Concentration protocol

(supplied with kit), centrifuge at 10,000 x g for 30 seconds at room temperature. Discard flowthrough and re-use the collection tube. 5. Place column in the same 2 ml collection tube from step 4 and add 500 ul RWB Wash Buffer diluted with absolute