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文献和实验Extraction of DNA using DNAzol® Reagent
the culture plate and gently pipet the lysate into an assay tube. Cell Pellets or Suspensions: Add 1 ml of DNAzol® Reagent to 1-3 × 107 cells, either in pellet or in suspension (volume 100 µl), pellet the cells and wash them with 0.9% NaCl. Pellet
using a competitive ELISA format. The mean background signal in the shown assay was indistinguishable. Together, a low background and a small coefficient of variation allow one to define a low detection limit2 , which is often set three standard
Assay of superoxide dismutase activity
can assay the activity of a single SOD isoform on the polyacrylamide gel and exclude the interference coming from non-SOD molecules in the tissue crude extract, which is not possible using the spectrophotometric method. Materials and Methods
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