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文献和实验Preparation of HeLa Nuclear Extracts
Promoter-specific in vitro transcription by RNA polymerase II is now achievable with cell extracts prepared from a variety of animal and plant sources (1 –3 ). For extracts prepared from mammalian cells, HeLa cells provide many advantages
Preparation of Hela Cell Nuclear and Cytosolic S100 Extracts for In Vitro Splicing
and splicing in whole-cell extracts and were fairly inefficient, because of the different optima for these two reactions (1 ,2 ). It was later shown that these reactions could be uncoupled (3 ,4 ), but obtaining discrete pre-mRNAs in useful amounts remained
Transfection of Adipocytes and Preparation of Nuclear Extracts
The uniqueness of the adipose cell type must be considered before choosing a procedure for gene transfer into mature adipocytes. Because adipocytes isolated from adipose tissue (AT) are filled with so many lipids that cells cannot be plated
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