Enzymatics特约代理商|N2050L 25 mM d

用ReadyMixTM PCR Reaction Mix进行拟南芥SSLP

用ReadyMix TM PCR Reaction Mix 进行拟南芥SSLP （32 个样品+3 个对照） 一、PCR 采用SIGMA REDTaq® ReadyMixTM PCR Reaction Mix提供的 试剂 （包括20 mM Tris-HCl, pH 8.3, 100 mM KCl, 3 mM MgCl2, 0.002% gelatin, 0.4 M dNTP

b-galactosidase Solution Assay

of 0.25 M Tris HCl pH 8.0. • Freeze thaw the cells 3 times by alternating between dry ice and 37°. • Spin for 15 minutes at 4° and transfer the supernatant to a new tube and hold on ice. • Mix the following reaction solution per sample:       264

Targeted Exon Sequencing by In‐Solution Hybrid Selection

Klenow fragment (3′→5′ exo) and Klenow buffer 10 mM dNTP mix 1 mM dATP