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上海玉博生物科技有限公司
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文献和实验thymidine 5′‐triphosphate (TTP, 8t O ). The crude sample can be analyzed on a Welchrom C18 (or Ultisil C18) reversed‐phase column (4.6 × 250 mm) measured at 260 nm at a flow of 1.0 mL/min using a linear gradient of 0% to 40% buffer B in 20 min. Buffer A: 20
can be circumvented by using state-of-the-art assay diluents, like LowCross® HRP-Stab, that are designed to block interferences during detection, it is easy to prevent some of these effects by careful selection and performance testing of the blocking reagent. BSA
Identification of Proteins in Complex Mixtures Using Liquid Chromatography and Mass Spectrometry
only Ammonium bicarbonate (e.g., J.T. Baker), for insoluble fractions only 100 mM Tris, pH 8.5/8 M urea (e.g., Sigma)
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