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文献和实验; if you have problems with it, contact me :-) Jim Hutchins hutchins@umsmed.edu http://www.umsmed.edu/~hutchins/ References Raineri, I., Moroni, C. and Senn, H.P. (1991). Improved efficiency for single-sided PCR by creating a reusable pool
Reverse Ligation Mediated RT -
sequencing gel which has been pre - heated to 50°C. Fractionate by electrophoresis. Standard detection procedure for your label type. 上一篇：Gene-specific RT-PCR 下一篇：Reverse Transcriptase-PCR
trouble-shooting should problems arise. rSAGE Diagram Methods Step 1. cDNA synthesis with modified oligodT primer BRS1 Prepare Total RNA and polyA+ (Approximately 2-5 ug is needed for good representation) as described in SAGE.
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