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上海玉博生物科技有限公司
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文献和实验-7 cells were transfected with CDK2 siRNA or a negative control siRNA using the indicated volumes of transfection agent per well. 48 hr after transfection, the cells were harvested and analyzed by real-time RT-PCR using TaqMan® Gene Expression Assays
. The latter pathway requireshomologous recombination (HR) between chromosomal DNAand the supplied foreign DNA stimulated by the local DNAcleavage, resulting in virtually any type of desired editing throughreplacement of native sequence by the designed HR template
Transfection of siRNA. Cos-7 cells were transfected with CDK2 siRNA or a negative control siRNA using the indicated volumes of transfection agent per well. 48 hr after transfection, the cells were harvested and analyzed by real-time RT-PCR using TaqMan® Gene
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