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文献和实验Nick Translation of DNA for CGH
of the two. We attempt to freeze down one lot of 10X Enzyme with known characterstics to be used for several months. DNA Pol-1/DNase I : this is the " slow enzyme" mix in the Nick Tranlsation Kit from GIBCO. You can also order the enzyme separately from Gibco
DNA labeling by nick translation
2.5 µl EDTA (0.5 M, pH 8.0) and 2.5 µl SDS (20%) to stop the reaction, keep the probes at -20℃ until hybridization Optimal fragment length after nick translation DNA after agarose gel
DNA labeling by nick translation
after addition of new DNAse and Pol -->add 2.5 µl EDTA (0.5 M, pH 8.0) and 2.5 µl SDS (20%) to stop the reaction, keep the probes at -20℃ until hybridization Optimal fragment length after nick translation DNA after agarose
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