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The ribonuclease protection assay (RPA)

, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32

Chromatin IP (CHIP assay)

and measure protein concentration (BioRad assay) use 1 - 5 mg protein per IP immunoprecipitate for 2 h to ON, 4 °C wash immunoprecipitations pelleting the beads each time:      2 x 1 ml CHIP lysis buffer      2 x 1 ml CHIP

How Do I make an Antibody against a Peptides

of mcKLH is highly soluble, making coupling reactions easier to complete. Figure 1. Peptide Carrier Conjugation using Maleimide Activated Carriers Figure 2. EDC-Mediated Peptide-Carrier Conjugation