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上海玉博生物科技有限公司
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文献和实验Collagen gel containing 3T3 fibroblasts (dermal equivalent for raft culture)
75?) 1.5mL 10x reconstitution buffer 1.5mL 10x DMEM 12mL rat tail type 1 collagen (>3.8mg/mL) 10N NaOH Glacial acetic acid (in case) Method Pre-chill pipettes
primary fibroblasts (˜ cell number 1 x 106 ) use: A. 25 ml rat tail collagen (approx conc 2mg/ml) B. 3 ml 10xMEM C. 0.22 M NaOH: first, add 2ml, then drop-wise whilst stirring, until the collagen turns
/ml denatured rat tail collagen (Vitrogen; Collagen Corporation). To prepare collagen-coated dishes: Dilute Vitrogen stock (~3 mg/ml) 1:100 in sterile PBS, then completely cover the surface of a 10cm dish with 3-5 ml. Incubate at 33
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