ZYMO RESEARCH特约代理商|T2001 EZ酵母转

Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions

Table of Contents Introduction Strategic Planning Basic Protocol 1: Bait Construction, Transformation into Yeast Reporter Strain

High‐Throughput Screening for Protein‐Protein Interactions Using Yeast Two‐Hybrid Arrays

Orr‐Weaver, T.L., Szostak, J.W., and Rothstein, R.J. 1981. Yeast transformation: A model system for the study of recombination. Proc. Natl. Acad. Sci. U.S.A. 78:6354‐6358.   

【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离

amount of cells,as low as 25 mL per transformation, works equally well. 6. Use of the S.O.C. medium is optional here, which contains 2%tryptone, 0.5% yeast extract, 2.5 mM KCl, 10 mM NaCl, 10 mMMgCl2, 10 mM MgSO4, and 20 mM glucose with pH adjustedto 7.0 ±