USBIO特约代理商|C2605 Cell Attachme

Biological coating procedures for glass coverslips

  COLLAGEN Collagen may be used to coat glass coverslips for the growth of epithelial, endothelial and muscle cells, neurons, PC12 and CHO cell lines. Type I collagen is most often used for coating substrates for cell culture

Extraction of DNA using DNAzol® Reagent

before extraction with DNAzol® Reagent. 2. Centrifugation (optional): Sediment the homogenate for 10 min at 10,000 × g at 4°C or room temperature. Following centrifugation, transfer the resulting viscous supernatant to a fresh tube. This step removes

Extraction of DNA using DNAzol Reagent

of cells and nuclei: 1) Cells grown in monolayer: Add 0.75-1.0 ml of DNAzol® Reagent per 10 cm2 culture plate area. Lyse the cells by agitating the culture plate and gently pipet the lysate into an assay tube. 2) Cell Pellets or Suspensions: Add