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文献和实验Monocytes/Macrophages: Enrichment of PBMCs with Monocytes
gradient (see below). Apply all the cells on the Percoll gradient. Centrifuge 30 min at 400 x g without brakes. Collect enriched PBMCs and transfer into a 50 ml tube. Fill the tube with PBS, centrifuge 10 min at 300 x g.
Enrichment of PBMCs with monocytes (The Science Advisory Board)
gradient (see below). 10. Apply all the cells on the Percoll gradient. 11. Centrifuge 30 min at 400 x g without brakes. 12. Collect enriched PBMCs and transfer into a 50 ml tube. 13. Fill the tube with PBS, centrifuge 10 min at 300 x g. 14
Isolation of PBMCs from patient blood and buffy coats
1| Transfer heparinized venous blood of patient to plastic50-ml tubes and dilute with an equal volume of PH buffer. When sodium citrate has been used as anticoagulant, add an equal volume of PT buffer. When PBMCs are isolated
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