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文献和实验-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
at 1:200 dilution) Cy3-conjugated affinity-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
. Be aware that the chosen protein must not react with any of the affinity reagents to be used (e.g. rabbit serum cannot be used with protein A gold; bovine serum (FCS) cannot be used as a blocking agent if anti-BSA labelling is being studied).
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