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Analysis of Proteins using Small Format 2D Gel Electrophoresis

twice with PBS-A and lysed in 0.2 ml of 2D lysis buffer (0.01M Tris-HCl, pH 7.4, 1mM EDTA, 8M urea, 0.05M dithiothreitol, 10% (v/v) glycerol, 5% (v/v) Nonidet P-40, 200µg/ml RNAse A, 6% (w/v) pH 3.5-10 carrier ampholytes ("Resolyte", Merck

One-Dimensional SDS and Non-Denaturing Gel Electrophoresis of Proteins with Novex® Tris-Glycine Pre-Cast Gels

SureLock™ Mini-Cell manual (IM-9003) for detailed instructions. Note: If you are using only one gel, the plastic Buffer Dam replaces the second gel cassette. 6)        Fill the Upper Buffer Chamber with a small amount of the running buffer to check

聊一聊血清学研究中的表面封闭剂

-Buffer® STRONG and incubate (2 h) 100 µl per well on the plate 8. Wash three times with 300 µl 1-fold Washing Buffer TRIS per well 9. Incubate (1 h) with detector conjugate stored in HRP-Protector™ 10. Detect with substrate after further washing