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文献和实验DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
of the reagent per 2 x 106 of cultured cells. 2) Cells Grown in Monolayer Lyse cells directly in a culture dish by adding 300 μL of Cell Lysis Buffer per 2 x 106 of cultured cells directly into each well of multiwell cell culture plate or flask
A general protocol for staining cell for cytometry analysis
as described below: *The optimal amount of PI may range between 2–10µl/test depending on cell type and experimental system. 2 µl/test is the recommended starting amount. 4.Gently mix the cells and incubate for 15 min at RT in the dark. *For Annexin V
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
9. GlutaMAX™-I Supplement 10. Basic FGF, Recombinant Human 11. β-Mercaptoethanol, 1000X 12. Penicillin-Streptomycin, Liquid 13. Attachment Factor 14. TrypLE™ Select Cell Dissociation Reagent or 0.05% Trypsin/EDTA
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