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文献和实验Expression of Eukaryotic Cytochromes P450 in E. coli
Although much has been learned from the study of relatively abundant cytochromes P450 (e.g., P450cam from Pseudomonas putida cytosol, P4501A2 and P4502B4 from rabbit liver microsomes, and P45Oscc from bovine adrenal cortex mitochondria
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
medium for OP9 cells (for Protocols 1, 2) Leukemia inhibitory factor (LIF; 10 µg/mL; Chemicon LIF2010) (for Protocol 2; see Step 20) Lympholyte-M (Cedarlane CL5120) (for Protocol 3) Mice, 4-8 wk old (for Protocol 4; see Step 69) Mice, fetal
Methods for the Detection of D-Amino-Acid Oxidase
crosslinker, Stratagene, La Jolla, CA). The membrane was incubated at 65°C for 1 hr in a prehybridization solution [5 x SSPE (1 x SSPE is 0.18 M NaCl, 10 mM sodium phosphate, and 1 mM EDTA, pH 7.7), 5 x Denhardt's solution (8 ), and 0.5% SDS] containing 20 μg
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