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- miltenyi biotec
- 130-048-101
- 2025年07月13日
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上海玉博生物科技有限公司
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文献和实验Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
Transfection Kit (Clontech) CD34+ cell separation kit (CD34 MicroBead Kit containing anti-human CD34 MicroBeads and Blocking Reagent; Miltenyi Biotec) Cellgro medium (serum-free medium; Cellgenix) DMEM for HSC Fetal calf serum (FCS
Mouse B cell isolation with magnetic beads
9.Constant volume to 20 ml/ each spleen with MACS, and transfer to another tube through a new strainer, centrifuging for 5 min, 4℃; Resuspend cells with MACS to 1 ml/ each spleen. 10.Follow Miltenyi Biotec protocol of column
Native Chromatin Preparation and Illumina/Solexa Library Construction
Antibody and preimmune serum for ChIP CD4+ T cell Isolation Kit II (human) (Miltenyi Biotec) Alternatively, Dynabeads Untouched Human CD4 T Cells Kit (Invitrogen) can be used (see Steps 5-21). DNA End-Repair Kit (Epicentre Biotechnologies
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