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文献和实验LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
. 4.3 mM CaCl2,1.2 M KI,50 mM Tris-HCl,pH 8.0,5 ml. 5.0.5 mM ATP,0.2 mM CaCl2,0.5 mM DTT,2 mM Tris-HCl,pH 8.0 at 4℃,1 liter. 6.CFSE (Molecular Probes,C-1311; not the diacetate),prepare a stock solution of 50 mg/ml in DMSO. 7.Small SS34 tubes and adaptors
that must be examined lack cytological detail. On the other hand, 5, 6 carboxy 2′,7′ dichloro-fluorescein diacetate succinimidyl ester (CCSFE), a carboxyfluorescein derivative that resists bleaching (5 ) better than the monochloro-form (1 ), is a stable cytoplasmic
Measurement of Cell Adhesion Under Static Conditions
5. During incubation load test cells with 6-carboxyfluorescein diacetate (6-CFDA) as follows: A. Wash cell suspension with RPMI 1640 B. Resuspend cells in 4.8 ml of RPMI 1640 C. Add 200 � of a 1 mg/ml solution of 6-CFDA dis- solved in RPMI 1640 D
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