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Models of Inflammation: Carrageenan‐ or Complete Freund Adjuvant (CFA)

1‐ml syringes Kimwipes Clear plastic box for the inhalational anesthesia or animal restrainer

Polyclonal and Monoclonal Antibodies

medium. 3) Resuspend in 1 ml of ice cold freezing solution. 4) Transfer vial to an insulated freezing box and place at -70 C for at least 1 hr. (could be for a couple of days). 5) Transfer the vial from the -70 C to the liquid nitrogen

Studies of the Ubiquitin Proteasome System

6.6 ) Basic Protocol 17: Radiolabeling Ubiquitin with 32P   Materials 20 mg/ml bovine ubiquitin (Sigma‐Aldrich) in coupling buffer Coupling buffer: 50 mM sodium borate, pH 8.5 10 mg/ml Sulfo‐NHS‐LC‐Biotin (Pierce