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上海玉博生物科技有限公司
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文献和实验Construction and Characterization of Adenovirus Vectors
before use. Equipment Agarose gel electrophoresis equipment Bottles (polypropylene, 500- and 1000-mL) Centrifuge (high-performance) (e.g., Avanti J-series, Beckman) Cryovials (4-mL) Dialysis cassettes (Slide-A-Lyzer, 10,000-kDa
Fluorescence Quenching Methods to Study Lipid‐Protein Interactions
and Measurement of Fluorescence Quenching Efficiency Materials 25 mg/ml dioleoylphosphatidylcholine (DOPC; Avanti Polar Lipids) in chloroform
. Centrifuge again in a tabletop centrifuge at 20,000g for 10 min at 4°C. 15. Pass the supernatant through a 0.45-µm filter into a precooled Falcon tube using a 10-mL syringe. Immediately add 5 µL of RNasin per 1 mL of extract
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