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文献和实验off. Sample Preparation 1) Prepare sample buffer by mixing 50 µL of NuPAGE Sample Buffer, 30 µL D.I. water, and 20µL of reducing agent. (Reducing agent can be either 0.5 M DTT in stabilized liquid form or beta-mercaptoethanol) 2) Add
NuPAGE Gel Electrophoresis 蛋白电泳
.Using a razor blade cut the sides of the gel.The thick edge at the bottom of the gel should be cut off.Sample Preparation1)Prepare sample buffer by mixing 50 µL of NuPAGE Sample Buffer,30 µL D.I.water,and 20µL of reducing agent.(Reducing agent
Sample Solublization Buffers for Two-Dimensional Electrophoresis
urea and 2 M thiourea), 2–4% nonionic and/or zwitterionic detergent(s), reducing agent(s), carrier ampholytes and, depending on the type of sample, protease inhibitors. In this chapter, the major constituents of sample solubilization/lysis buffers
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