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High‐Throughput Cytotoxicity Screening by Propidium Iodide Staining

) 0.05 mg/ml propidium iodide (PI) in phosphate‐buffered saline (PBS; appendix 2A ): 800 µl per plate Compounds for cytotoxicity testing (1 to 10 mM in DMSO) in a stock

Genome Size Determination Using Flow Cytometry of Propidium Iodide-Stained Nuclei

. Propidium iodide (PI) staining with flow cytometry is a powerful method for genome sizing because it is relatively fast, works with a wide variety of materials, and provides information on a very large number of nuclei. In this method, nuclei are stained

The Use of Propidium Iodide to Assess Excitotoxic Neuronal Death in Primary Mixed Cortical Cultures

in a variety of these pathologies. A key to testing such drugs is the use of a fast, reliable, and easily reproducible model of neurodegeneration and neuroprotection. Our laboratory has previously used propidium iodide (PI) to assess