Kapa biosystems 特约代理商|KK7251 K

Dynabeads® mRNA DIRECT Kit

by reverse transcriptase (RT)-PCR from highly specialized cells (e.g. isolated from a heterogenous sample by immunomagnetic separation). In addition, the protocol has been successfully used to isolate mRNA from a wide variety of tissues of mammalian, fish

【原创】血液中直接PCR,无需DNA提纯

文库里全部的变异株，那么就要建造一个更大的，并且很昂贵的实验室。这在目前的情况下，完全不现实。这个瓶颈问题可以很容易地通过KAPA Biosystems公司的乳状液膜技术（Emulsion Technology）来实现。其技术核心是在一个1ml的试管中，将含有表面活性剂和膜溶剂的油相和水相(内水相) 置于容器中,在高速搅拌下制成油包水型乳状液,再将此乳状液分散到另一种水溶液(第3 相)中, 就得到了水包油再油包水型乳状液膜。当乳状液分散到第3 相时,形成数亿万记的乳珠。这样，每个乳珠就变成了一个小的PCR反应

Mapping, PCR DNA Sequencing

Software for: Making Restriction Maps Designing PCR primers Assembling fragments, detecting SNPs, and managing raw sequence data For:GCG,Mac,PC,UNIX,and on the Web Restriction Mapping -Making restriction maps was my first use