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文献和实验LINEAGE ANALYSIS USING RETROVIRAL VECTORS
can be found elsewhere [46]. For either avian or murine retroviruses, the overall strategy is the same. A population of double-stranded DNA molecules that includes a short degenerate region, [(G or C)(A or T)]12, is generated by PCR amplification of a chemically constructed
Studies of the Ubiquitin Proteasome System
) Kit for first‐stand cDNA synthesis (e.g., RT‐PCR kit; Fermentas) Kit for PCR amplification of DNA (e.g., RT‐PCR kit, Fermentas, or Ready‐To‐Go PCR beads, GE Healthcare) Primers for PCR
to these methods, Albertsen et al., of Pioneer Hi-Bred, U.S. Pat. No. 5,432,068, have developed a system of nuclear male sterility which includes: identifying a gene which is critical to male fertility; silencing this native gene which is critical to male fertility
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