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文献和实验Endothelial Cell Systems - Instructions For Use
results, donor information and the number of cells contained in the cryovial. 2. Endothelial Cell Growth Medium (EGM®), as either: Endothelial Cell Growth Medium (EGM®) , (CC-3024), a complete medium in a 500 ml bottle with attached Bovine Brain
medium (MCDB-131 medium with 5 ml of 200 mM L-glutamine, 10% FBS and EGM-2 SingleQuots) at 37 °C, 5% CO2 . 5) Slowly change medium every 24 hours for the first 7 days, then every other day. EPC colonies can be identified after an average
PriCells: Isolation, culture, and purification of Human Pulmonary Microvascular Endothelial Cells
on 6-well plates pre-coated with gelatin (0.2%) for several days until subconfluent. 8. Purification: Endothelial cells were isolated using magnetic beads (Dynal) according to the instructions of the manufacturer. 9. The medium was replaced every 2 to 3 days
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