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- 2025年07月07日
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文献和实验Preparation Of Ciliated Protozoa For Scanning Electron Microscopy
fume hood. Supplies and most EM chemicals can be obtained from Electron Microscope Sciences, Ernest F. Fullum, Ladd Research Industries, Polysciences, Ted Pella, and Bio-Rad. Instant fixation Materials: Polypropylene
Purification of dnEBNA-1/Soft from E. coli BL21 LysS
per 0.5L culture Incubate 2-3hrs 37℃250rpm Spin down 2 flasks of each (1L total)in 500ml GSA centrifuge bottles 5000rpm,4℃ 10min Decant supernatant,freeze pellet @20℃ Resuspend pellet in TED+0.15M NaCl (2-5ml per gram wet weight of pellet; for bacterial
) we have had little luck with counterstains, but have found them unnecessary with phase optics. Other plastics can be easily substituted. See the Ted Pella catalog for a number of epoxy alternatives. How to make durcupan. 1. Buy a kit from Fluka (cat
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