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文献和实验Enrichment of PBMCs with monocytes (The Science Advisory Board)
gradient preparation: 1. Mix in 50 ml tube*: 13.5 ml Percoll (Amerhsam), 15.0 ml MEM Spinner modification (Sigma), 1.5 ml 10x Earles salts solution (Biochrom) 2. Centrifuge 10 min at 14000 x g without breaks in a fixed angle rotor. 3. Carefully
The Cell Environment (including types of culture medium)
PBS, Hanks BSS, Earles saltsBalanced salt solutions DPBS (Prod. No. D8537 / D8662) Form the basis of many complex media HBSS (Prod. No. H9269 / H9394) EBSS (Prod. No. E2888)Basal media
The Cell Environment (including types of culture medium)
Examples Uses Balanced salt solutions PBS, Hanks BSS, Earles salts DPBS (Prod. No. D8537 / D8662 ) HBSS (Prod. No. H9269 / H9394 ) EBSS (Prod. No. E2888 ) Form the basis
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